Part 1. Identify cell features through microscopy and cellular features: For each micrograph, describe the type of microbe as:
(A) Bacteria, Virus, or Protozoa (you will not be able to identify genus or species from these images; do not use a reverse image search to find answers)
(B) Describe the cell features that led you to this answer
(C) Include cellular features that can be identified from this image. Think about what you can tell about the cell from the image.
3. Dark-field micrograph (1000x) 4. Gram Stain: Light Microscope (1000x)
2. Endospore stain: Light Microscope (1000x)
1 Trichrome Stain: Light Microscope (100x)
5. TEM (see bar for sizing)
Part 2. Growing bacteria in the lab: For each of the following bacteria, discuss the conditions that would be needed for growth in the lab. Include all the physical and chemical requirements discussed in the lecture slides.
1. Escherichia coli
2. Clostridium botulinum
3. An environmental bacteria isolated from marine sediments from the Massachusetts coastline in March
4. Mycobacterium tuberculosis
Part 3. Constructing a growth curve: using the following data (you can draw the curve or produce a graph on excel or google sheets). Make sure to label the x- and y-axis, and the different stages of the growth curve. You need to account for the dilution (do not just use the numbers provided).
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